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. 1980 Feb;39(2):141–149.

Studies of efferent lymph cells from nodes stimulated with oxazolone.

J G Hall, J Hopkins, J Reynolds
PMCID: PMC1457959  PMID: 7380464

Abstract

Efferent lymph from nodes regional to areas of skin that had been treated with solutions of oxazolone in acetone was collected from unanaesthetized sheep. The application of 5% solutions of oxazolone to unsensitized sheep caused no signs of acute inflammation or 'shut-down' of lymphocyte traffic; none the less, normal immune responses ensued so that immunoblasts, some containing immunoglobulin, were discharged into the lymph together with specific humoral antibodies. When previously sensitized sheep were challenged with 2.5% solutions of oxazolone the vigorous secondary responses were heralded by Arthus reactions, induced presumably by pre-existing antibodies, which were mainly of the IgG class. A similar sequence of events occurred in a thymus-deprived sheep which had undergone intra-uterine thymectomy at 60 days of gestation. Repeated applications of oxazolone to normal sheep did not exhaust or inhibit the characteristic changes in the flow and composition of the lymph. When immunoblasts from efferent lymph were radiolabeled with 125I-UdR and returned intravenously to the sheep they showed no significant tendency to localize either specifically or non-specifically in areas of skin that had been treated with contact-sensitizing chemicals.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alexander P., Bensted J., Delorme E. J., Hall J. G., Hodgett J. The cellular immune response to primary sarcomata in rats. II. Abnormal responses of nodes draining the tumour. Proc R Soc Lond B Biol Sci. 1969 Nov 18;174(1035):237–251. doi: 10.1098/rspb.1969.0090. [DOI] [PubMed] [Google Scholar]
  2. Asherson G. L., Allwood G. G. Inflammatory lymphoid cells. Cells in immunized lymph nodes that move to sites of inflammation. Immunology. 1972 Mar;22(3):493–502. [PMC free article] [PubMed] [Google Scholar]
  3. Asherson G. L., Allwood G. G., Mayhew B. Contact sensitivity in the mouse. XI. Movement of T blasts in the draining lymph nodes to sites of inflammation. Immunology. 1973 Sep;25(3):485–494. [PMC free article] [PubMed] [Google Scholar]
  4. Askenase P. W., Asherson G. L. Contact sensitivity to oxazolone in the mouse. VIII. Demonstration of several classes of antibody in the sera of contact sensitized and unimmunized mice by a simplified antiglobulin assay. Immunology. 1972 Sep;23(3):289–298. [PMC free article] [PubMed] [Google Scholar]
  5. Birbeck M. S., Hall J. G. Transformation, in vivo, of basophilic lymph cells into plasma cells. Nature. 1967 Apr 8;214(5084):183–185. doi: 10.1038/214183a0. [DOI] [PubMed] [Google Scholar]
  6. Cole G. J., Morris B. The growth and development of lambs thymectomized in utero. Aust J Exp Biol Med Sci. 1971 Feb;49(1):33–53. doi: 10.1038/icb.1971.4. [DOI] [PubMed] [Google Scholar]
  7. Davies A. J., Carter R. L., Leuchars E., Wallis V. The morphology of immune reactions in normal, thymectomized and reconstituted mice. II. The response to oxazolone. Immunology. 1969 Jul;17(1):111–126. [PMC free article] [PubMed] [Google Scholar]
  8. HALL J. G., MORRIS B. The lymph-borne cells of the immune response. Q J Exp Physiol Cogn Med Sci. 1963 Jul;48:235–247. doi: 10.1113/expphysiol.1963.sp001660. [DOI] [PubMed] [Google Scholar]
  9. HALL J. G., MORRIS B. The output of cells in lymph from the popliteal node of sheep. Q J Exp Physiol Cogn Med Sci. 1962 Oct;47:360–369. doi: 10.1113/expphysiol.1962.sp001620. [DOI] [PubMed] [Google Scholar]
  10. Hall J. G. A method for collecting lymph from the prefemoral lymph node of unanaesthetised sheep. Q J Exp Physiol Cogn Med Sci. 1967 Apr;52(2):200–205. doi: 10.1113/expphysiol.1967.sp001902. [DOI] [PubMed] [Google Scholar]
  11. Hall J. G., Birbeck M. S., Robertson D., Peppard J., Orlans E. The use of detergents and immunoperoxidase reagents for the ultrastructural demonstration of internal immunoglobulin in lymph cells. J Immunol Methods. 1978;19(4):351–359. doi: 10.1016/0022-1759(78)90019-4. [DOI] [PubMed] [Google Scholar]
  12. Hall J. G., Hopkins J., Orlans E. Studies on the lymphocytes of sheep. III. Destination of lymph-borne immunoblasts in relation to their tissue of origin. Eur J Immunol. 1977 Jan;7(1):30–37. doi: 10.1002/eji.1830070108. [DOI] [PubMed] [Google Scholar]
  13. Hall J. G., Morris B., Moreno G. D., Bessis M. C. The ultrastructure and function of the cells in lymph following antigenic stimulation. J Exp Med. 1967 Jan 1;125(1):91–110. doi: 10.1084/jem.125.1.91. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hall J. G., Morris B. The immediate effect of antigens on the cell output of a lymph node. Br J Exp Pathol. 1965 Aug;46(4):450–454. [PMC free article] [PubMed] [Google Scholar]
  15. Hall J. G. Studies of the cells in the afferent and efferent lymph of lymph nodes draining the site of skin homografts. J Exp Med. 1967 May 1;125(5):737–754. doi: 10.1084/jem.125.5.737. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Hay J. B., Morris B. Generation and selection of specific reactive cells by antigen. Br Med Bull. 1976 May;32(2):135–140. doi: 10.1093/oxfordjournals.bmb.a071345. [DOI] [PubMed] [Google Scholar]
  17. McConnell I., Lachmann P. J., Hobart M. J. Restoration of specific immunological virginity. Nature. 1974 Jul 12;250(462):113–116. doi: 10.1038/250113a0. [DOI] [PubMed] [Google Scholar]
  18. Moore A. R., Hall J. G. Nonspecific entry of thoracic duct immunoblasts into intradermal foci of antigens. Cell Immunol. 1973 Jul;8(1):112–119. doi: 10.1016/0008-8749(73)90098-1. [DOI] [PubMed] [Google Scholar]
  19. OORT J., TURK J. L. A HISTOLOGICAL AND AUTORADIOGRAPHIC STUDY OF LYMPH NODES DURING THE DEVELOPMENT OF CONTACT SENSITIVITY IN THE GUINEA-PIG. Br J Exp Pathol. 1965 Apr;46:147–154. [PMC free article] [PubMed] [Google Scholar]
  20. Rose M. L., Parrott D. M., Bruce R. G. Migration of lymphoblasts to the small intestine. II. Divergent migration of mesenteric and peripheral immunoblasts to sites of inflammation in the mouse. Cell Immunol. 1976 Nov;27(1):36–46. doi: 10.1016/0008-8749(76)90151-9. [DOI] [PubMed] [Google Scholar]
  21. Scollay R., Hall J., Orlans E. Studies on the lymphocytes of sheep. II. Some properties of cells in various compartments of the recirculating lymphocyte pool. Eur J Immunol. 1976 Feb;6(2):121–125. doi: 10.1002/eji.1830060210. [DOI] [PubMed] [Google Scholar]

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