Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1980 Nov;41(3):643–651.

Properties of peritoneal exudate lymphocytes that mediate tuberculin delayed-type hypersensitivity and anti-tuberculosis immunity.

M J Lefford
PMCID: PMC1458151  PMID: 7461706

Abstract

Rats were immunized with living BCG and acute peritoneal exudates were induced on the ninth day of infection. The peritoneal exudate cells (PEC), that confer adoptive anti-tuberculosis immunity and tuberculin delayed-type hypersensitivity (DTH), were subject to velocity sedimentation analysis. It was found that the ability to confer immunity of DTH was limited to a population of cells that sedimented at a rate of 3-4 mm/h. This sedimentation rate corresponds to that of small lymphocytes. No significant immunological activity was detected in large lymphocytes that incorporate [3H]-thymidine in vitro, regardless of whether the exudates were obtained 14 to 24 h after induction of peritoneal inflammation. The failure of large lymphocytes to confer immunity and DTH was not due to adherent cells with suppressor activity, because removal of adherent cells failed to amplify the transfer of immunological activity by non-adherent cells. The persistence of the ability to express immunity and DTH in adoptively immunized rats was studied. There was no decay of adoptive immunity during a 4 week period following cell transfer, but there was a rapid reduction in the expression of DTH.

Full text

PDF
643

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ambrose C. T., Donner A. Application of the analysis of variance to hemagglutination titrations. J Immunol Methods. 1973 Oct;3(2):165–209. doi: 10.1016/0022-1759(73)90031-8. [DOI] [PubMed] [Google Scholar]
  2. GOWANS J. L. The fate of parental strain small lymphocytes in F1 hybrid rats. Ann N Y Acad Sci. 1962 Oct 24;99:432–455. doi: 10.1111/j.1749-6632.1962.tb45326.x. [DOI] [PubMed] [Google Scholar]
  3. Golstein P., Blomgren H. Further evidence for autonomy of T cells mediating specific in vitro cytotoxicity: efficiency of very small amounts of highly purified T cells. Cell Immunol. 1973 Oct;9(1):127–141. doi: 10.1016/0008-8749(73)90174-3. [DOI] [PubMed] [Google Scholar]
  4. Hunt S. V., Ellis S. T., Gowans J. L. The role of lymphocytes in antibody formation. IV. Carriage of immunological memory by lymphocyte fractions separated by velocity sedimentation and on glass bead columns. Proc R Soc Lond B Biol Sci. 1972 Sep 19;182(1067):211–231. doi: 10.1098/rspb.1972.0076. [DOI] [PubMed] [Google Scholar]
  5. Koster F. T., McGregor D. D. The mediator of cellular immunity. 3. Lymphocyte traffic from the blood into the inflamed peritoneal cavity. J Exp Med. 1971 Apr 1;133(4):864–876. doi: 10.1084/jem.133.4.864. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kostiala A. A., Lefford M. J., McGregor D. D. Immunological memory in tuberculosis. 2. Mediators of protective immunity, delayed hypersensitivity and macrophage migration inhibition in central lymph. Cell Immunol. 1978 Nov;41(1):9–19. doi: 10.1016/s0008-8749(78)80024-0. [DOI] [PubMed] [Google Scholar]
  7. Kostiala A. A., McGregor D. D., Lefford M. J. The mediator of cellular immunity. XI. Origin and development of MIF producing lymphocytes. Cell Immunol. 1976 Jun 15;24(2):318–327. doi: 10.1016/0008-8749(76)90215-x. [DOI] [PubMed] [Google Scholar]
  8. Lefford M. J., McGregor D. D. Immunological memory in tuberculosis. I. Influence of persisting viable organisms. Cell Immunol. 1974 Dec;14(3):417–428. doi: 10.1016/0008-8749(74)90192-0. [DOI] [PubMed] [Google Scholar]
  9. Lefford M. J., McGregor D. D., Mackaness G. B. Immune response to Mycobacterium tuberculosis in rats. Infect Immun. 1973 Aug;8(2):182–189. doi: 10.1128/iai.8.2.182-189.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lefford M. J., McGregor D. D., Mackaness G. B. Properties of lymphocytes which confer adoptive immunity to tuberculosis in rats. Immunology. 1973 Oct;25(4):703–715. [PMC free article] [PubMed] [Google Scholar]
  11. Lefford M. J., McGregor D. D. The lymphocyte mediators of delayed hypersensitivity: the early phase cells. Immunology. 1978 Apr;34(4):581–590. [PMC free article] [PubMed] [Google Scholar]
  12. Lefford M. J. Properties of peritoneal exudate lymphocytes that mediate tuberculin delayed-type hypersensitivity and anti-tuberculosis immunity. I. The effect of cytotoxic agents. Immunology. 1980 Nov;41(3):635–642. [PMC free article] [PubMed] [Google Scholar]
  13. Lefford M. J. The measurement of tuberculin hypersensitivity in rats. Int Arch Allergy Appl Immunol. 1974;47(4):570–585. doi: 10.1159/000231251. [DOI] [PubMed] [Google Scholar]
  14. MacDonald H. R., Phillips R. A., Miller R. G. Allograft immunity in the mouse. II. Physical studies of the development of cytotoxic effector cells from their immediate progenitors. J Immunol. 1973 Aug;111(2):575–589. [PubMed] [Google Scholar]
  15. Marchal G., Milon G., Hurtrel B., Lagrance P. H. Titration and circulation of cells mediating delayed type hypersensitivity in normal and cyclophosphamide treated mice during response to sheep red blood cells. Immunology. 1978 Dec;35(6):981–987. [PMC free article] [PubMed] [Google Scholar]
  16. McGregor D. D., Logie P. S. The mediator of cellular immunity. VI. Effect of the antimitotic drug vinblastine on the mediator of cellular resistance to infection. J Exp Med. 1973 Mar 1;137(3):660–674. doi: 10.1084/jem.137.3.660. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. McGregor D. D., Logie P. S. The mediator of cellular immunity. VII. Localization of sensitized lymphocytes in inflammatory exudates. J Exp Med. 1974 Jun 1;139(6):1415–1430. doi: 10.1084/jem.139.6.1415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Miller R. G., Phillips R. A. Separation of cells by velocity sedimentation. J Cell Physiol. 1969 Jun;73(3):191–201. doi: 10.1002/jcp.1040730305. [DOI] [PubMed] [Google Scholar]
  19. Moore A. R., Hall J. G. Nonspecific entry of thoracic duct immunoblasts into intradermal foci of antigens. Cell Immunol. 1973 Jul;8(1):112–119. doi: 10.1016/0008-8749(73)90098-1. [DOI] [PubMed] [Google Scholar]
  20. Rieke W. O., Schwarz M. R. The types of rat thoracic duct lymphocytes which respond to phytohemagglutinin in vitro. Acta Haematol. 1967;38(2):121–128. doi: 10.1159/000209008. [DOI] [PubMed] [Google Scholar]
  21. Schlossman S. F., Levin H. A., Rocklin R. E., David J. R. The compartmentalization of antigen-reactive lymphocytes in desensitized guinea pigs. J Exp Med. 1971 Sep 1;134(3 Pt 1):741–750. doi: 10.1084/jem.134.3.741. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sprent J., Miller J. F. Fate of H2-activated T lymphocytes in syngeneic hosts. III. Differentiation into long-lived recirculating memory cells. Cell Immunol. 1976 Feb;21(2):314–326. doi: 10.1016/0008-8749(76)90059-9. [DOI] [PubMed] [Google Scholar]
  23. Stutman O. Two main features of T-cell development: thymus traffic and postthymic maturation. Contemp Top Immunobiol. 1977;7:1–46. doi: 10.1007/978-1-4684-3054-7_1. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES