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. 2000 Dec 12;98(1):283–288. doi: 10.1073/pnas.011454198

Figure 1.

Figure 1

Steady-state levels of wild-type and mutant β-lactamases from a wild-type strain of E. coli (XL1-Blue). Lane 1, control of purified wild-type β-lactamase; lanes 2, 4, 6, and 8, whole cell extracts; lanes 3, 5, 7, and 9, periplasmic extracts; lanes 2 and 3, wild-type; lanes 4 and 5, L76N; lanes 6 and 7, M182T; and lanes 8 and 9, L76N/M182T. Total β-lactamase was determined from whole-cell extracts boiled in sample buffer; periplasmic extracts were prepared as described in Materials and Methods.