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Effects of expression in a protease-deficient strain. (A) XL1-Blue (wild-type strain); (B) SB646 (protease-deficient strain). Active and inactive β-lactamases within the periplasmic space of a protease-deficient strain. The amount of active β-lactamase was calculated from the activity of periplasmic extracts and the specific activity of the purified protein. The total amount of β-lactamase protein was determined from densitometry of Western blots (see text).
