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. 2006 May 5;34(8):2355–2363. doi: 10.1093/nar/gkl277

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© The Author 2006. Published by Oxford University Press. All rights reserved

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An elongated uORF decreases TPO mRNA expression. (A) Schematic representation of mutated TPO minigenes. The positions of the respective termination codons (Ter) are indicated. Arrows are indicating the length of the respective mRNAs. Nonsense codon positions are counted from the (Met) at position 1. (B) Northern blot of cytoplasmic RNA from HeLa cells cotransfected with either the TPO minigene wt (lane 3) or mutants (ΔG, lane 4; ΔATG 7, lane5; ΔIntron 4, lane 6; NS40 ΔG, lane 7; NS40 ΔG ΔIntron 4, lane 8; ΔG ΔIntron 4, lane 9; NS40 ΔATG7, lane 10; NS26 ΔG, lane 11; NS28 ΔG, lane 12) together with the control plasmid. Cytoplasmic RNA from untransfected HeLa-cells (lane 1) or cells transfected with only the control plasmid (lane 2) is shown. Expression of the mRNAs was analyzed as described in Figure 1B. Percentages and standard deviations (SD) are based on three independent experiments.