Figure 5.

Electrophysiological properties of HrpZ_Psph-induced currents. (A) HrpZ_Psph-induced currents do not exhibit rectifying properties. After addition of HrpZ_Psph to the cis compartment of the bilayer cuvette, ion currents were recorded at a membrane potential of −100 mV and after immediately switching the membrane potential to +100 mV after 2 sec of total recording. (B) Linear current–voltage relationship of HrpZ_Psph-induced ion fluxes. Current–voltage relationships of HrpZ_Psph-induced nonselective cation currents were established in three different electrolyte solutions: 100 mM KCl and 10 mM Hepes, pH 7.0 (○); 100 mM NaCl and 10 mM Hepes, pH 7.0 (▫); and 90 mM K⁺-gluconate, 10 mM KCl, and 10 mM Hepes, pH 7.0 (▵). The means of three recordings are given.