Table 1.

Binding^* of HrpZ_Psph to TRANSIL beads coated with single lipid membrane bilayers

Membrane composition	Bound protein (% of total)
1,2-diacyl-sn-glycero-3-phosphocholine	55  ± 5
POPC/POPE (80∶20)	80  ± 5
1,2-di(trans-9-octadecenoyl)-sn-glycero-3-  phosphocholine/1,2-ditetradecanoyl-sn-  glycero-3-[phospho-rac-(1-glycerol)] (98∶2)	95  ± 5

^*Binding of HrpZ_Psph to TRANSIL beads coated with single lipid membrane bilayers was performed as described in Materials and Methods. Total protein applied, lipid-bound, and unbound protein were separated by SDS/PAGE, and Coomassie-stained proteins were quantified by light densitometry.