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. Author manuscript; available in PMC: 2006 May 8.
Published in final edited form as: J Am Chem Soc. 2003 Oct 22;125(42):12668–12669. doi: 10.1021/ja036155z

Figure 2.

Figure 2

Binding of 125I-HgTX1A19Y/Y37F to Kv1.3 channels. Membranes prepared from HEK293 cells stably transfected with the Kv1.3 channel were incubated with ∼0.13 pM 125I-HgTX1A19Y/Y37F, in the absence or presence of increasing concentrations of (left panel) compound 3 (solid green circles), compound 4 (solid red triangles), compound 6 (solid black tilted squares), compound 7 (solid blue squares), compound 13 (open magenta squares); (right panel) compound 5 (solid blue triangles), compound 8 (solid black triangles), compound 9 (solid pink circles), compound 10 (solid red tilted squares), compound 11 (open green circles), compound 12 (solid magenta squares), for 20 h at room temperature. Inhibition of binding was assessed relative to an untreated control. Specific binding data can be fit to a single-site inhibition model, as described in the Supporting Information.