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. 2000 Dec 26;98(1):295–300. doi: 10.1073/pnas.011524298

Figure 1.

Figure 1

DA reduces the unitary EPSP in layer V pyramidal neuronal pairs in the ferret PFC. (A) Schematic of experimental protocol used for dual whole-cell recording in current clamp mode and to show the method of DA application. The reconstructed neurons shown were simultaneously recorded and filled with Lucifer yellow and biocytin, respectively. (B) Example of a presynaptic action potential eliciting a unitary EPSP in a postsynaptic neuron. (C) Neurolucida reconstruction of a reciprocally connected pair of neurons. The dendritic arbor of one cell is drawn in light blue and its axonal arborization in purple, whereas the dendritic arbor of the other neuron is drawn in red and its axons in green. Three green dots indicate putative synaptic contacts established by green axons on light blue dendrites. The purple dot indicates the synaptic contact formed by purple axons on red dendrites. (Scale bar, 100 μm.) (D) The pressure application of 10 mM DA acutely depresses EPSP amplitude. (E) Histogram showing DA reduction of EPSP amplitude normalized to control values. EPSP amplitude is significantly reduced by 28.9 ± 0.29% from control levels (n = 16, P < 0.001).