Table 2.
Immunoreactivity of the notochord and its sheath to the applied primary antibodies.
| Antibody | Notochord | Notochordal attachment to the sheath | outer /middle part of the sheath |
|---|---|---|---|
| Anti-Fish Collagen Type I | + | + | + + +/+ + + |
| Anti-Human Alpha-Smooth Muscle Actin | + + + | + + + | - |
| β-tubulin (D-10) | - | - | + + +/+ |
| Anti-Human Cytokeratin MNF116 | - | - | + + +/ - |
| Anti-Human Cytokeratin 20 | - | - | + + +/+ + + |
| Anti-Human Cytokeratin 7 | - | - | - /+ + |
| Anti-Human Cytokeratin LP34, 34βE12, 35βH11 | - | - | - /+ + + |
| Anti-Human Cytokeratin 8 | - | - | + +/ - |
| Anti-Human Desmin | - | - | + + +/+ |
| Anti-Vimentin/HRP | - | - | + + +/+ |
| Anti-Human GFAP | - | - | - |
| Anti-Human Neurofilament | - | - | + + +/ + |
| Anti-Human Nestin | - | - | - |
+++ = very strong immunoreactivity, ++ = strong immunoreactivity, + = mild immunoreactivity, - = no immunoreactivity