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. Author manuscript; available in PMC: 2006 May 9.
Published in final edited form as: Int J Radiat Oncol Biol Phys. 2006 Apr 1;64(5):1482–1494. doi: 10.1016/j.ijrobp.2005.11.045

Fig. 5.

Fig. 5

Improved apoptosis induction and activation of the apoptotic signal transduction pathway by the combination of TRAIL gene therapy and radiotherapy but not in NHLF in vitro. A, FACS analysis was used to quantify apoptotic cells 4 days after 1 of 6 treatments in NHLF. B, FACS was used to quantify apoptotic cells 4 days after 1 of 6 treatments in Seg-1 cells. C, Western blot analysis of TRAIL expression and cleavage of PARP, caspase-8, caspase-9, and caspase-3 in Seg-1 cells 4 days after Ad/TRAIL-F/RGD (TRAIL) treatment combined with radiotherapy. Cells were treated with PBS (lane 1), Ad/CMV-GFP (GFP; MOI of 500 VP) (lane 2), Ad/TRAIL-F/RGD (MOI 500 VP) (lane 3), radiotherapy (2 Gy) (lane 4), Ad/CMV-GFP (MOI 500 VP) plus radiotherapy (2 Gy) (lane 5), and Ad/TRAIL-F/RGD (MOI of 500 VP) plus radiotherapy (2 Gy) (lane 6). ß-Actin was used as a loading control. P<0.05 in Figure 5B as comparison between Ad/TRAIL-F/RGD plus radiation and PBS, Ad/CMV-GFP, Ad/TRAIL-F/RGD, radiation, Ad/CMV-GFP plus radiation respectively and combined.