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Calcium-induced apoptosis of cerebellar granule neurons from knockout mice, grown in high KCl. Cells from normal (+/+) and mutant (−/−) mice were cultured on coverslips as described in 55 mM KCl for 5 days, then fixed and stained with ApopTag kit. Two fields for each treatment are shown. High KCl induced apoptosis in knockout but not normal neurons.
