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The morphology of hESCs cultured in the presence of feeder cells (MEF), conditioned medium (CM), or HESCO. The morphology of H9 (Upper) and BG01 (Lower) cells cultured on the feeder cells (Left) and in HESCO (Right) are more condensed with a high nucleus/cytoplasm ratio, whereas the cells cultured in the conditioned medium (Center) are elongated with a low nucleus/cytoplasm ratio. (Original magnification, ×40.)
