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. 2006 Apr 4;103(15):5688–5693. doi: 10.1073/pnas.0601383103

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© 2006 by The National Academy of Sciences of the USA

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Fig. 2. — Expression of stem cell markers in hESCs cultured in HESCO. (A) Alkaline phosphatase assay of hESCs cultured in the presence of mouse embryonic fibroblast feeder cells (MEF), conditioned medium (CM), or HESCO was performed. (Magnification, ×100.) (B) (Left) Immunofluoresence staining with antibodies to stem cell markers: Oct4, SSEA3, SSEA4, TRA-1-60 (Tra 60), TRA-1-81 (Tra 81), or control mouse IgM (Ig). Mouse IgG and rat IgM controls show results similar to those of mouse IgM control (data not shown). (Center) The nuclei were stained with DAPI. (Right) The overlay of FITC antibody staining and DAPI signals is shown in the column labeled Merge. (Magnification, ×200.)