Fig. 2.

Fabrication of immunological synapse arrays. (A) Schematic procedure of surface patterning. A PNMP thin film is irradiated through a photomask (Ai), developed with PBS (Aii), and then re-exposed to UV in the absence of a mask (Aiii). A first ligand is deposited (Aiv), followed by washing with pH 7.4 buffer (Av), and finally the second ligand is immobilized by using the newly exposed biotin groups (Avi). (Aiv and Avi Insets) The structure of protein ligand linkages to the surface. The chemical structure of the PNMP photoresist and film after UV exposure and development (Aii) is further illustrated in Fig. 7. (B) Fluorescence micrographs taken from one field of a two-component patterned surface. SAv-Texas red detecting biotinylated α-CD3 (Left), α-ICAM-1-FITC detecting ICAM-1 (Center), and their overlay (Right).