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. 2006 Apr 3;103(15):5746–5751. doi: 10.1073/pnas.0509935103

Fig. 2.

Fig. 2.

Stepping trace and step-size histogram of GFP–DmVIIa–HMM with FIONA. (A and B) Stepping traces of GFP–DmVIIa–HMM molecules with double (A) and single (B) GFP signals. Values are the step sizes (in nm) between dwell periods. The experiment was performed at 0.5 μM ATP. (C and D) The horizontal red lines are fit to single Gaussian curves. (C) Step sizes: two GFP molecules; 32.7 ± 16.9 nm (mean ± SD); R = 97; n = 71 steps from 10 GFP–DmVIIa–HMM molecules. (D) Step sizes: one GFP molecule; 59.9 ± 20.5 nm (mean ± SD); R = 0.85; n = 17 steps from 32 GFP–DmVIIa–HMM molecules.