Fig. 4.
High molecular weight aggregates of mutant SOD1 in spinal microsomal fractions. (A) Age-dependent increase of high molecular weight SOD1 species in SOD1G93A spinal microsomal fractions in overexposed immunoblot of spinal microsomal fractions of SOD1G93A mice. Asterisks indicate high molecular weight species. (B) Quantification of high molecular weight species over the course of the disease relative to the ER resident protein calnexin by optical density analysis. Values represent means ± SEM (n = three per group). ∗, P < 0.05; one-way ANOVA, Student–Newman–Keuls method.