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. 2006 Apr 4;103(15):6025–6030. doi: 10.1073/pnas.0509227103

Fig. 5.

Fig. 5.

Mutant SOD1, but not hSOD1WT or mSOD1, interacts with BiP in the microsomal fraction in vivo. (A) Immunoprecipitation with an anti-BiP antibody followed by immunoblot using anti-BiP and anti-SOD1 antibodies. (B) Immunoprecipitation with an anti-BiP antibody using spinal microsomal fractions of SOD1G93A mice at different disease stages and nontransgenic mice. Input represents 10% of protein from spinal microsomal fraction. (C) Immunoprecipitation with an anti-BiP antibody followed by immunoblot using anti-BiP, -SOD1, and -ATF6 antibodies. (D) High molecular weight (HMW) SOD1 species in microsomal fractions interact with BiP. (E) Immunoprecipitated with/without anti-BiP antibody followed by a size-exclusion filter assay.