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. 2006 Mar 17;103(13):4988–4993. doi: 10.1073/pnas.0600083103

Fig. 1.

Fig. 1.

Retroviral expression of Chx10 in neonatal rats promotes bipolar cells at the expense of photoreceptors. (A) Schematic diagram of retroviral vector pMXIE. Flag-tagged Chx10 was subcloned upstream of the internal ribosome entry site-eGFP cassette. (B) Clones expressing the empty vector (MXIE; green) contained mostly photoreceptors (Upper), whereas clones expressing Chx10 (Lower) contained less photoreceptors and more bipolar cells. (Scale bar: 10 μm.) (C) Chx10 expression does not affect clone size (P > 0.05). (D) Chx10 increases the proportion of bipolar to total GFP+ cells >5-fold at the expense of photoreceptors. Error bars represent SD. Asterisks indicate significant difference from control (P ≤ 0.001). O, ONL; I, INL; Am, amacrine cells; BiP, bipolar cells; GCL, ganglion cell layer; Mü, Müller cells; PR, photoreceptor cells.