Fig. 3.

Presence of mutated Chx10 mRNA in photoreceptor and glial-like cells in the or^J retina. (A) In the WT retina (Top), Chx10 mRNA (red) is expressed mainly in bipolar cells in the INL. Most Müller glia (Cralbp protein; green) do not express Chx10. In the Chx10^−/− or^J retina, however, mutated Chx10 mRNA is expressed in the majority of Cralbp⁺ cells (Middle). The proportion of glia expressing Chx10 declines when proliferation is partially rescued in the Chx10^−/−;p27^Kip1−/− retina (Bottom). Yellow brackets indicate the position of Müller glia cell bodies in the INL as opposed to processes that also label with Cralbp. (B) Expression of Chx10 mRNA (red) in photoreceptors (rod arrestin protein; green) is extremely rare in the WT (Top) but is detected in the or^J retina (Middle) and is even more frequent in the Chx10^−/−;p27^Kip1−/− retina (Bottom). Images to the far right in A and B show enlarged views of the boxed areas. (C) Quantification of the fraction of cells expressing Chx10 mRNA and rod arrestin protein in the WT, or^J, or Chx10^−/−;p27^Kip1−/− retina. Error bars represent SD. Asterisks indicate significant difference (P ≤ 0.05) from WT (Chx10^−/−) or Chx10^−/− (Chx10^−/−;p27^Kip1−/−) retina. (Scale bars: A and B, 10 μm.) O, ONL; I, INL.