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. 2006 Mar 17;103(13):4988–4993. doi: 10.1073/pnas.0600083103

Fig. 5.

Fig. 5.

ShRNA-mediated acute Chx10 knockdown blocks bipolar cell differentiation and promotes photoreceptor cell differentiation. (A) P0 mouse retina was electroporated with scrambled shRNA (Upper) or Chx10 shRNA (Lower) vectors together with a GFP vector to mark transfected cells (green). Chx10 shRNA reduced the proportion of bipolar cells in the INL (yellow brackets) and increased the proportion of photoreceptors in the ONL (white brackets). (B) Quantification of cell-types in mature retina after electroporation at P0. Both Chx10 shRNA vectors (Chx10-shRNA-1-12, Chx10-shRNA-2-4) significantly reduced the proportion of bipolar cells and increased the proportion of photoreceptors. ShRNA-resistant human Chx10 vector reversed the effects of Chx10 shRNA. Human Chx10 plus a scrambled shRNA vector increased bipolar cell production. Error bars represent SD. Asterisks indicate significant difference from the empty vector control (P ≤ 0.05). (C) Chx10 shRNA has no significant effect on proliferation (P > 0.05). BrdU was injected 2 h before death, and incorporation was measured at P3 and P8 in cells expressing scrambled shRNA or Chx10 shRNA.