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Northern blots and real-time miRNA-PCR validation of miRNA chip data in CD34 progenitor differentiation experiments. (A) Northern blots for miR-223, miR-130a, and miR-10a. Loading RNA control was performed with U6. (B) miRNA RT-PCR for miR-10a, miR-106, miR-126, and miR-130a. The miRNA expression is presented as fold difference with respect to CD34+ cells before culture. (C and D) Temporal array expression of miR-223, miR-15–A, and miR-16-1 by miRNA RT-PCR.
