Fig. 2.

MAFB is a target of miR-130a. (A) MAFB mRNA and protein expression in CD34⁺ progenitors induced to megakaryocytic differentiation. β-Actin was used for RT-PCR and Western blot loading controls. (B) Relative repression of luciferase activity in MEG01 cells cotransfected with miR-130A and PGL3 3′UTR MAFB, PGL3 WT, and miR-130 seed match mutated. As a control scramble oligo sequences were cotransfected with PGL3 3′UTR MAFB. (C) Western blotting of MAFB total protein lysates in K562 cells transfected with miR-130a and scramble.