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. 2006 Mar 20;103(13):5078–5083. doi: 10.1073/pnas.0600587103

Fig. 3.

Fig. 3.

MiR-10a down-regulates HOXA1 by mediating RNA cleavage. (A) RT-PCR for HOXA1 gene expression in differentiated megakaryocytes (relative amount of transcript with respect to CD34+ progenitors at baseline). (B) hoxa1 protein expression in differentiated megakaryocytes. (C) Relative repression of luciferase activity of HOXA1 3′ UTR cloned in PGL3 reporter plasmid when cotransfected with miR-10a and control scramble. (D) Complementarity between miR-10a and the HOXA1 3′UTR as predicted by pictar. (E) Northern blotting for miR-10a gene expression in scramble and miR-10a precursor transfected K562 cells. (F) RT-PCR for HOXA1 gene expression in scramble and miR-10a precursor transfected K562 cells. (G) Western blotting for HOXA1 in K562 cells transfected with control scramble and precursor miR-10a.