Table 1.

miRNAs down-regulated during in vitro CD34⁺ megakaryocytic differentiation

miRNA	Chromosomal location	t test*	Fold change	Putative targets
hsa-mir-010a†	17q21	−9.10	50.00	HOXA1, HOXA3, HOXD10, CRK, FLT1
hsa-mir-126†	9q34	−2.73	8.33	CRK, EVI2, HOXA9, MAFB, CMAF
hsa-mir-106†	xq26.2	−2.63	2.86	TAL1, FLT1, SKI, RUNX1, FOG2, FLI, PDGFRA, CRK
hsa-mir-010b†	2q31	−2.17	11.11	HOXA1, HOXA3, HOXD10, ETS-1, CRK, FLT1
hsa-mir-130a†	11q12	−2.08	4.76	MAFB, MYB, FOG2, CBFB, PDGFRA, SDFR1, CXCL12
hsa-mir-130a-prec†	11q12	−2.07	7.69	NA‡
hsa-mir-124a	8q23	−1.81	2.78	TAL1, SKI, FLT1, FOG2, ETS-1, CBFB, RAF1, MYB
hsa-mir-032-prec	9q31	−1.76	3.57	NA‡
hsa-mir-101	1p31.3	−1.75	3.33	TAL1, CXCL12, MEIS1, MEIS2, ETS-1 RUNX1, MYB
hsa-mir-30c	6q13	−1.71	2.56	CBFB, MAFG, HOXA1, SBF1, NCOR2, ERG
hsa-mir-213†	1q31.3	−1.69	2.38	MAX-SATB2
hsa-mir-132-prec	17p13	−1.67	4.17	NA‡
hsa-mir-150†	19q13.3	−1.63	5.26	MYB, SDFR1
hsa-mir-020	13q31	−1.62	2.17	TAL1, SKI, RUNX-1, FLT1, CRK, FOG2, RARB
hsa-mir-339	7p22	−1.60	3.03	SKI, ETV6, GATA2, FLT1, RAP1B, JUNB, MEIS2
hsa-let-7a	9q22	−1.58	2.94	HOXA1, HOXA9, MEIS2, ITGB3, PLDN
hsa-let-7d	9q22	−1.56	2.17	HOXA1, HOXD1, ITGB3, RUNX1, PDGFRA
hsa-mir-181c	19p13	−1.55	2.50	RUNX-1, KIT, HOXA1, MEIS2, ETS-1 ETV6, PDGFRA
hsa-mir-181b	1q31.3	−1.53	2.13	RUNX-1, KIT, ITGA3, HOXA1, MEIS2, ETS-1, SDFR1.
hsa-mir-017	13q31	−1.38	1.82	TAL1, SKI, FLT1, RUNX1, CRK, FOG1, ETS-1, MEIS1

All differentially expressed miRNAs have q value <0.01 (false-positive rate).

*t test P < 0.05.

^†These miRNAs were identified by PAM as predictors of a megakaryocytic class with the lowest misclassification error. All, except miR-143, are down-regulated during megakaryocytic differentiation.

^‡miRNA precursor sequence that not contain the mature miRNA, therefore no putative target is shown.