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Secondary structure predictions of AVP-binding sequences. Site-directed modification of 157-B4 with a hexaethylene glycol linker (PEG18) led to a series of intermediate sequences with a PEG18 linker located in the AU-rich region (e.g., F-11). In the final sequence NOX-F37, the AU-rich region was completely removed, the secondary structure was opened at this site and closed with the PEG18 linker at the former 5′ and 3′ end.
