Fig. 5.
SP increases IL-8 mRNA and protein expression in human mesenteric preadipocytes. (A) ELISA assays were performed on supernatants from human mesenteric preadipocytes treated with SP for 4 h with or without prior CJ 012,255 treatment for 20 min. SP addition caused a significant increase in the secretion of IL-8 by these cells, an effect that was abolished by the specific NK-1R inhibitor CJ 012,255 (n = 8 per group). (B) Real-time PCR analysis of RNA extracts from the same cells produced similar data. (C) When the cells were exposed to two different concentrations of SP, maximum IL-8 mRNA expression was achieved at a dose of 10−8 M (n = 6 per group). (D) In cells treated with SP for various time intervals (1, 2, 4, 6, and 24 h), maximum IL-8 mRNA expression was observed after 4 h of treatment (n = 6 per group).