Fig. 2.

Vps46p interaction with Vps4pE233Q is enhanced and ATP independent. (A) Protein extracts were prepared from WT, vps4Δ, vps32Δ, and vta1Δ (KEBY88, KEBY92, KEBY112, and JLY03) yeast expressing Vps46p–HA (pJL11). GST pull-downs were performed as described in Fig. 1 A and B except using GST–Vps4p (positive control) and GST–Vps4pE233Q. (B) In vitro binding analysis was performed as in Fig. 1C except Vps46p–6XHIS was incubated with glutathione Sepharose and GST alone (negative control), GST–Vps4p (positive control), or GST–Vps4pE233Q.