Fig. 3.

Inverse correlation of myeloid and erythroid transcription factors upon restoration of C/EBPα expression in bcr/abl-expressing cells. (A) Northern blot of four independent clones of K562αER cells (14) treated with vehicle alone (−) or with 1 μM β-estradiol (+) for 48 h. The blots were hybridized to probes detecting the granulocytic marker C/EBPε and the erythroid marker GATA-1. 18S rRNA was used as a loading control for GATA-1. (B) Western blot of K562αER cells at various time points after treatment with 1 μM β-estradiol. GATA-1 and Id1 were detected by using nuclear extracts. C/EBPε was detected by using trichloroacetic acid extracts. A rapid and strong up-regulation of Id1 was detected.