Skip to main content
. 2006 Apr 14;103(17):6512–6517. doi: 10.1073/pnas.0601682103

Fig. 1.

Fig. 1.

Su48 is expressed in a variety of tissues and cancer cell lines. (A) RT-PCT analysis of Su48 expression. cDNA samples were prepared from various mouse tissues (1, heart; 2, brain; 3, spleen; 4, lung; 5, liver; 6, skeletal muscle; 7, testis; 9–12, whole embryos of day 7, 11,15, and 17, respectively; 13, no cDNA; 14, mouse embryonic day 11 cDNA library). One nanogram of each sample was used. The housekeeping gene glyceraldehyde-3-phosphate dehydrogenase (G3PDH) was used as control to confirm that the amount of total cDNA used in each reaction was comparable. (B) RT-PCT analysis of Su48 expression in human cell lines. cDNA was prepared from the following human cell lines: PANC-1, 293T, HBL100, HeLa, and U2OS. (C) Su48 protein expression in various cancer cell lines. Endogenous Su48 protein was evaluated using affinity-purified polyclonal anti-Su48 antibody (Upper). To confirm that similar amounts of protein were loaded in each lane, the membrane was reprobed with an anti-α-tubulin antibody (Lower). WB, Western blotting.