Fig. 7.

Microinjection of anti-Su48 antibody causes polyploidy. (A) HeLa cells were microinjected with a mouse monoclonal anti-Su48 antibody (24F1) or an isotype-matched control antibody. Twenty-four hours after injection, cells were analyzed by immunostaining with Cy3-conjugated anti-mouse antibody (red). At least 200 cells were injected and analyzed for each sample. (B) Quantification of the effect of anti-Su48 antibody on cell division after microinjection. The percentage of cells with two or more nuclei is shown.