Fig. 1.
Netrin-1 stimulates endothelial cell nitric oxide (NO•) production. Confluent endothelial cells were incubated with different concentrations of netrin-1 (10, 30, and 100 ng/ml) at 37°C for 60 min in modified Krebs/HEPEs buffer containing the NO•-specific spin trap Fe2+(DETC)2. Cells were then gently collected for analysis of NO• production by using electron spin resonance (ESR). Data are presented as mean ± SEM. (A) Representative ESR spectra. (B) Grouped data from six independent experiments. ANOVA; ∗, P < 0.05; ∗∗, P < 0.01.