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DCC is specifically required for netrin-1 activation of eNOS. (A) Representative ESR spectra. (B) Grouped data of netrin-1 stimulated nitric oxide (NO•) production from cells transfected with control siRNA (25 nmol/liter) or DCC siRNA (25 nmol/liter). Detailed transfection procedure is included in Experimental Procedures. Data are presented as mean ± SEM. ANOVA; ∗, P < 0.01 vs. control siRNA with netrin-1; #, P < 0.01 vs. control siRNA without stimulation.
