Fig. 2.

Expression and purification of CARDS TX protein. (Upper) Distribution of UGA codon within mpn372. The eight TGA codons within the coding region of CARDS TX were modified into TGG codons (at nucleotide positions shown in the schematic diagram) to express in E. coli. (Lower) CARDS TX gene was cloned in pET19b vector and expressed in E. coli BL21(DE3). Recombinant His-10-tagged protein was purified by using nickel affinity column chromatography and eluted by imidazole. Proteins were resolved in 4–15% gradient SDS/PAGE gel. Lane 1, overexpressed rCARDS TX in E. coli BL21(λDE3); lane 2, purified rCARDS TX.