Fig. 2.

TLM-deficient viral particles are trapped in the endosome. PDH were inoculated with WT or mutant DHBV (200 MGE). After 10 h of incubation with WT DHBV, DHBVD1/2, DHBVD2, and DHBVD1, cells were harvested and subfractionated. Cytosolic and endosomal fractions were adjusted to identical protein concentrations, and their purity was controlled by immunoblotting by using grb2 (cytoplasm)- and clathrin HC (endosomes)-specific antisera. For detection of viral DNA in the cytosolic (c) and endosomal (e) fractions, TaqMan PCR was performed. The y axis indicates the number of viral genomes per 25 μl of resuspended subcellular fraction.