Fig. 1.

T. brucei contains two eukaryotic TrpRSs. (A) Multiple sequence alignment of the cytosolic TrpRS from S. cerevisiae (ScWRS1) and the two T. brucei orthologues (Tb-TrpRS1 and Tb-TrpRS2). The sequences were aligned by using the clustal w program with default parameters. Strictly conserved residues and conservative replacements are shown in black and gray boxes, respectively. (B) Position of the two trypanosomal enzymes Tb-TrpRS1 (Tb1) and Tb-TrpRS2 (Tb2) in a phylogenetic tree based on a multiple sequence alignment of the cytosolic TrpRS from mouse [Mus musculus (Mm)], Drosophila melanogaster (Dm), and yeast [S. cerevisiae (Sc)]; the mitochondrial enzyme of yeast (Sc mito) and mouse (Mm mito); and, indicated by gray letters, the TrpRS from the bacteria Corynebacterium glutamicum (Cg), Bacillus subtilis (Bs), and E. coli (Ec). The tree was constructed by using treeview, which is available on http://taxonomy.zoology.gla.ac.uk/rod/treeview.html.