Fig. 4.

Ablation of Tb-TrpRS2 selectively abolishes OXPHOS. Succinate and α-ketoglutarate mitochondrial ATP production in crude mitochondrial fractions of the Tb-TrpRS2 RNAi cell line. Uninduced cells (−Tet) are shown on the left, and induced cells (+Tet) are shown on the right of the graphs. The substrate tested is indicated at the top, and additions of antimycin (antim.) and atractyloside (atract.) are indicated at the bottom. ATP productions in mitochondria isolated from uninduced cells tested without antimycin or atractyloside are set to 100%. The bars represent means expressed as percentages from three or more independent inductions. SEs are indicated.