Fig. 4.

Kinetics of DME and ROS1 action on 5-meC and thymine in CpG and non-CpG sequence contexts. The time-dependent generation of incised oligonucleotides was measured by incubating purified DME or ROS1 with double-stranded oligonucleotide substrates containing a 5-meC·G pair (•) or a T·G mismatch (○) in CpG (CXGG) and non-CpG (XCGG, XAG, and AXT) sequence contexts. Reactions were stopped at the indicated times, products were separated in a 12% denaturing polyacrylamide gel, and the relative amount of incised oligonucleotide was quantitated by phosphor imaging.