Fig. 4.
Relative curvature of the 1HN chemical shifts as a function of temperature in the absence of GuHCl. (A) Relative curvature plotted versus residue number. The curvature is normalized to the curvature of the chemical shifts for E42, which shows the largest deviation from linearity. The graph includes only those residues for which the chemical shift changes are significantly better represented by a second-order polynomial than a first-order polynomial (P < 0.05, F test). (B) The relative curvature mapped onto the structure of ACBP (Protein Data Bank ID code 1NTI). The data are color-coded from 0 (yellow) to 1 (red).