Table 1.
Genes selected for validation by in situ hybridization from the full list of genes on the MOE430A chip more abundantly expressed in Ret+/+ versus Retk−/k− intestine
| Gene symbol | Gene name | Fold change WT/MUT | Unigene |
|---|---|---|---|
| Mapk10 | Mitogen-activated protein kinase 10 | 89.88 | Mm.39253 |
| Sncg | Synuclein, γ | 59.42 | Mm.282800 |
| Mab21i1 | Mab-21-like 1 | 51.19 | Mm.252244 |
| Prph1 | Peripherin | 33.04 | Mm.2477 |
| Ascl1 | Achaete-scute complex homolog-like 1 | 30.99 | Mm.136217 |
| Stmn3 | Stathmin-like 3 | 29.01 | Mm.2319 |
| Tubb3 | Tubulin, β3 | 28.96 | Mm.40068 |
| Dlx1 | Distal-less homeobox 1 | 25.29 | Mm.4543 |
| Serpini1 | Serine (or cysteine) peptidase inhibitor, clade I, member 1 | 20.19 | Mm.41560 |
| Elavl4 | ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D) | 18.01 | Mm.3970 |
| Pcdha | Protocadherin α | 17.28 | Mm.308500 |
| Phox2a | Paired-like homeobox 2a | 16.83 | Mm.358574 |
| Vip | Vasoactive intestinal polypeptide | 16.17 | Mm.98916 |
| Mapt | Microtubule-associated protein τ | 16.05 | Mm.1287 |
| Elavl2 | ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B) | 15.16 | Mm.318042 |
| Stmn2 | Stathmin-like 2 | 14.37 | Mm.29580 |
| Sox10 | SRY-box containing gene 10 | 12.53 | Mm.276739 |
| Sox2 | SRY-box containing gene 2 | 10.97 | Mm.4541 |
| Uchl1 | Ubiquitin carboxyl-terminal hydrolase PGP9.5 | 10.77 | Mm.29807 |
| Dcx | Doublecortin | 10.72 | Mm.12871 |
| Tagln3 | Transgelin 3 | 9.968 | Mm.24183 |
| Ebf3 | Early B cell factor 3 | 9.837 | Mm.258708 |
| Cart | Cocaine and amphetamine regulated transcript | 9.657 | Mm.75498 |
| Nsg2 | Neuron-specific gene family member 2 | 8.049 | Mm.3304 |
| Gap43 | Growth-associated protein 43 | 7.615 | Mm.1222 |
| Hmx3 | H6 homeobox 3 | 7.291 | Mm.323562 |
| Syt11 | Synaptotagmin XI | 7.061 | Mm.379376 |
| Scg3 | Secretogranin III | 6.567 | Mm.2386 |
| Arhgef3 | Rho guanine nucleotide exchange factor 3 | 6.191 | Mm.248606 |
| Phox2b | Paired-like homeobox 2b | 6.098 | Mm.62505 |
| Gng3 | Guanine nucleotide binding protein (G protein), γ3 subunit | 5.623 | Mm.329700 |
| Hoxb5 | Homeobox B5 | 5.298 | Mm.207 |
| Hoxd4 | Homeobox D4 | 5.219 | Mm.1214 |
| Dpysl3 | Dihydropyrimidinase-like 3 | 5.181 | Mm.8180 |
| Tmeff2 | Transmembrane protein with EGF-like and two follistatin-like domains 2 | 4.936 | Mm.245154 |
| Fgf13 | Fibroblast growth factor 13 | 4.827 | Mm.7995 |
| Crmp1 | Collapsin response mediator protein 1 | 4.386 | Mm.290995 |
| Gng2 | Guanine nucleotide-binding protein (G protein), γ2 subunit | 3.988 | Mm.41737 |
| Mllt11 | Myeloid/lymphoid or mixed-lineage leukemia (tri-thorax homolog Drosophila) translocated to 11 | 4.222 | Mm.331208 |
| Igsf4a | Immunoglobulin superfamily, member 4A | 3.42 | Mm.234832 |
| Cdh2 | Cadherin 2 | 3.279 | Mm.257437 |
| L1cam | L1 cell adhesion molecule | 2.807 | Mm.260568 |
| Gfra1 | Glial cell line-derived neurotrophic factor family receptor α1 | 2.772 | Mm.88367 |
| Tgfb2 | Transforming growth factor, β2 | 2.326 | Mm.18213 |
| Ret | Ret proto-oncogene | 1.913 | Mm.57199 |
| Etv1 | Ets variant gene 1 | 1.885 | Mm.4866 |
| Tbx3 | T-box 3 | 1.812 | Mm.219139 |
Genes from the full list of genes on the MOE430A chip more abundantly expressed in Ret+/+ versus Retk−/k− intestine (Table 3) were selected for validation by RNA in situ hybridization on tissue sections (Fig. 1). In this table, we identify those genes by (i) gene symbol, as assigned by the National Center for Biotechnology Information (NCBI), (ii) gene name as assigned by NCBI; (iii) fold change in Ret+/+ versus Retk−/k− intestine; and (iv) Unigene number. Previously undescribed ENS marker genes are in bold.