Fig. 4.

Effects of Of’br dsRNA on growth of the antenna, leg, and wing. (A and B) Both antennae (A) and legs (B) of uninjected nymphs (filled circles) grew steadily throughout the nymphal instars and increased by a factor of 1.5 at each molt (Insets). “Interval” in Insets indicates the growth that occurs between the two indicated stadia. Injection into early fourth-instar nymphs of dsRNA made from Acd’br (filled squares) or Of’br (open squares) had no effect on the growth trajectory of legs or antennae. (C and D) Effect of Of’br dsRNA on the width of the wing pad base (C) and the wing pad diagonal (D). Standard error is shown where the width of the bars is greater than the data points. Between 23 and 31 measurements were made for each Of’br dsRNA mean; between 5 and 7 measurements were performed for each Acd’br dsRNA mean. (E) Ethidium gel showing RT-PCR of Of’br mRNA in day-4 fourth-instar body, T2 legs, antennae, and wing pads. Data are typical of two determinations.