Fig. 2.

Association of the 4q35 locus with the nuclear matrix in normal cells and in primary myoblasts derived from FSHD patients. (A) Map of recombinant DNA used for hybridization with nuclear matrix DNA (E, EcoRI; K, KpnI; H, HincII). (B) Electrophoretic pattern of restriction fragments. Numbers indicate the positions of the restriction fragments on the map in A. (C) Hybridization with total DNA. The blot is underexposed to reveal DNA repeats. (D–G) Hybridization with nuclear matrix-associated DNA from HeLa cells, nuclear matrix-associated DNA from GM10115A cells (E), nuclear matrix-associated DNA from normal human myoblasts (F), and nuclear matrix-associated DNA from myoblasts derived from an FSHD patient (G). Arrows indicate the positions of the human and murine c-myc S/MARs used as controls, the FRR MAR, and the pLAM.