Fig. 3.

Mapping the nuclear matrix attachment site in the 4q35 locus by DNA array technique. (A and B) Hybridization of the total human DNA (A) and total DNA from the mouse–human hybrid cell line GM11015A (B) with a genomic DNA array covering 250 kb in the 4q35 locus. (C) The results of hybridization of nuclear matrix-associated DNA from cultured primary myoblasts from FSHD patients (white columns) and normal subjects (black columns). The hybridization data were normalized against a positive control. Hybridization to a S/MAR from the human c-myc gene locus in A and C and against murine c-myc S/MAR in B was assigned a value of 1. The average of three independent experiments (two hybridizations per experiment) is presented.