Fig. 2.

Effects of UCS15A. (A) Chemical structures of UCS15A and its derivative, 2b. (B–E) Inhibition of AMAP1/cortactin and other SH3/proline bindings in vitro. The effects of UCS15A and 2b on the in vitro binding of GST-cortactin with GFP-AMAP1 (B) or GFP-dynamin2 (C) or the binding of GST-AMAP1 (SH3) with GFP-paxillin (D) and GST-Src (SH3) with myc-c-Cbl (E) were analyzed as in Fig. 1, in which GST beads were preincubated with the chemical compounds as described in refs. 9 and 10. (F) The effects of UCS15A and 2b on Matrigel transinvasion (Upper) and cell viability (Lower) of MDA-MB-231 and 4T1/luc cells were analyzed as in Fig. 1F. The mock included dimethyl sulfoxide (DMSO) used as a solvent. Results shown are the means ± SEM of three experiments. (G) (Upper) Effects of UCS15A on lung metastasis of 4T1/luc cells were measured as in Fig. 1G (red lines represent median values). (Lower) Tumor weights at the originally injected fat pad on day 19 also are shown. More than 20 mice were used for each experiment. Error bars show SEM.