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. 2006 Apr 25;103(18):7082–7087. doi: 10.1073/pnas.0510843103

Fig. 1.

Fig. 1.

Diagram of the plant transformation vectors pSW3 and pSW2. Shown are the genetic elements in pGPTV-Kan between the left border (LB) and right border (RB) sequences that define the transferred DNA. I: npt II, the neomycin phosphotransferase gene for kanamycin resistance, flanked by the nopaline synthase promoter (NOS) and the nopaline synthase polyadenylation signal (Ag7). II: the toxoid stxA2PO, flanked by the cauliflower mosaic virus (CaMV) 35S RNA promoter, followed by the 5′ untranslated region (UTR) from tobacco mosaic virus (TMVΩ) and the soybean vegetative storage protein (VSP) 3′ polyadenylation sequence. III: an intergenic terminator sequence derived from the potato proteinase inhibitor II gene, pin2. IV: the plant-optimized stxB2PO (pSW3) or native stxB2 (pSW2) eukaryotic expression cassette with the tobacco etch virus (TEV) 5′ UTR. Restriction sites used for ligations are indicated below the diagram.