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Western blot analysis of the Stx2 holotoxoid expressed by transformed NT-1 clones. The positive control consisted of 10 ng of the wild-type Stx2 purified from E. coli DH5α that harbored pMJ100 (39) (lane 1). Sonic lysates of 50 mg of fresh plant cells were used to load lanes 2–4. Lane 2, NT-1KanR clone served as the negative control; lane 3, NT#2 clone; and lane 4, NT#3 clone. Stx2A and Stx2B subunit positions are indicated by arrows.
