Table 5.
Effect of pair of agonists compared with single agonist in thrombin generationparameters (n = 30) using low doses agonists and rTF. Final concentration of agonists: ADP 0.2 μM; sodium arachidonate (AA) 0.125 mmol/L; collagen (Col) 0.05 μg/mL, recombinant tissue factor (rTF) 2.5 pmol/L
| B | C | D | E | |
| Lag Time (min.) mean ± SD | Time to Peak (min.) mean ± SD | PTG (nmol/L) mean ± SD | AUC (nmol/L) mean ± SD | |
| 1 ADP | 15.4 ± 4.0 | 23.2 ± 4.9 | 155 ± 41 | 1260 ± 474 |
| 2. AA +ADP | 11.3 ± 2.5 | 17.3 ± 2.6 | 198 ± 45 | 1604 ± 188 |
| 3. Col | 14.3 ± 3.3 | 21.6 ± 3.7 | 148 ± 44 | 1392 ± 264 |
| 4. AA+Col | 11.8 ± 2.5 | 17.8 ± 3.3 | 187 ± 53 | 1551 ± 257 |
| 5. AA | 12.9 ± 3.1 | 19.0 ± 3.4 | 179 ± 58 | 1488 ± 286 |
| 6. rTF | 5.8 ± 1.8 | 12.9 ± 2.4 | 154 ± 50 | 1495 ± 337 |
Parameters of TG in PRP from 30 normal volunteers. PTG: Peak to thrombin generation. AUC: Area under the curve. AA, ADP or Col were compared with their combining effects. Also rTF, 2.5 pM was compared with the other agonists.
Lag Time: 1 vs 2 <0.001; 3 vs 4 <0.01; 2 vs 5 = NS; 4 vs 5 = NS 6 vs 1 to 5 <0.001
Time to Peak 1 vs 2 <0.001; 3 vs 4 <0.001; 2 vs 5 = NS; 4 vs 5 = NS 6 vs 1 to 5 <0.001
PTG 1 vs 2 <0.001; 3 vs 4 <0.01; 2 vs 5 =NS; 4 vs 5 =NS 6 vs 1 to 5 =NS
AUCo→35min 1 vs 2 <0.001; 3 vs 4 =NS; 2 vs 5 =NS; 4 vs 5 =NS 6 vs 1 to 5 =NS
rTF appears as more effective than each agonist alone or their combination in shorten times parameters of TG, but effects are not different when amount parameters are considered.