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HTLV-I antisense transcription initiates in the 3' LTR. (A) 5'RACE analysis was conducted using RNA samples from 293T cells transfected with the K30-3'/5681 proviral DNA construct. The resulting amplified products were run on an agarose gel. M = 100 bp marker (the asterisk indicates the 600 bp band). (B) Position of the identified CAP sites for antisense transcripts are depicted in the 3' LTR. Nucleotide numbering corresponds to the sense strand.
