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Heterodimerization with κ receptors affects the level of β2 receptor-mediated phosphorylation of pMAP kinase. Cells expressing δ-β2 or κ-β2 receptors were treated with indicated concentrations of isoproterenol (A) or etorphine (B) for 5 min. The extent of phospho-MAP kinase (pMAPK) was determined by Western blotting analysis with antiphospho-MAP kinase antibody; standardization was with tubulin measured in the same blots, with the use of antitubulin antibody.
