Figure 4.

Glutathione peroxidase mimic ebselen inhibits estrogen-induced DNA synthesis. Cellular proliferation in the control and the treated cultures was measured by colorimetric immunoassay based on BrdU incorporation into the cellular DNA. Pretreatment with the glutathione peroxidase mimic ebselen showed a significant reduction of E2-induced DNA synthesis in endothelial cells. Data from three independent experiments are presented as ROS production with controls set at 100% (± SD). Values that are significantly different from E2 treatment alone (P < 0.05) are indicated with an asterisk (*). Significant increases in DNA synthesis by E2 (P < 0.05) are indicated by (#).